advances in proteomics analytical techniques

Authors

reyhaneh farrokhi yekta proteomics research center, shahid beheshti university of medical sciences

mehdi koushki clinical biochemistry department, school of medicine, tehran university of medical sciences, tehran, iran

nasrin amiri dashatan proteomics research center, shahid beheshti university of medical sciences

abstract

proteins are fundamental components of cells which mediate many essential biological processes. proteomics is a rapidly growing field for the study of proteome, the protein complement expressed by the genome of an organism or cell type. the large-scale analysis of proteins leads to a more comprehensive view of molecular and cellular pathways that improves the overall understanding of the complex processes supporting the living systems. the analysis of proteome is significantly challenging due to high dynamic range and difficulties in assessment of low abundance proteins and the absence of efficient purification and identification techniques. a variety of methods have been utilized for protein studies including gel-based techniques, protein microarrays, mass spectrometry-based approaches such as maldi and seldi, high and ultra-performance liquid chromatography and fourier transform ion cyclotron resonance mass spectrometry. nmr spectroscopy and x-ray crystallography methods are also used for structural study of proteins. this review aims to give a brief overview of some of the above techniques and their most recent advances. we also introduce proteominer, a recent protein enrichment technology for the exploration of the entire proteome content

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Journal title:
journal of paramedical sciences

جلد ۶، شماره ۳، صفحات ۰-۰

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